Rapid communication: the ovine cDNA encoding interferon-stimulated gene product 17 (ISG17).

Name of the Sequence. Ovine interferon-stimulated gene product 17 (oISG17). Genus and Species, Breed. Ovis aries, Purebred Romanov and Hampshire, crossbred Columbia × Rambouillet and Romanov × Hampshire. Origin of the Clone. Ovine endometrial RNA was pooled from nonpregnant and pregnant ewes (d 7, 8, and 10 nonpregnant; d 13, 15, and 16 pregnant and nonpregnant; and d 21 pregnant) and used to make a cDNA library that was cloned into a HybriZAP 2.1 twohybrid vector system (Stratagene, LaJolla, CA). Insert size ranged from .4 to 1.7 kb, with an average size of .9 kb. Bovine (b)ISG17 cDNA (Austin et al., 1996a) was radiolabeled (α-labeled 32P-dCTP, ≥ 3,000 Ci/mmol, Amersham Pharmacia Biotech, Piscataway, NJ) using a random prime reaction (Life Tech, Grand Island, NY). Phage from the ovine endometrial cDNA library were screened with radiolabeled bISG17 cDNA using standard procedures (Austin et al., 1996a). Positive plaques were isolated using autoradiography and then purified. Two positive clones were excised from phage and cDNA inserts were sequenced using ABI Prism dRhodamine Terminator Cycle Sequencing Ready Reaction procedures (Applied Biosystems, Foster City, CA). Cycle sequencing was performed using an Ericomp thermocycler (San Diego, CA) and the following conditions for 25 cycles: 96°C for 30 s, 50°C for 30 s, and 60°C for 4 min. Sequence Data. Both 614-bp oISG17 cDNA were identical. The ISG17 cDNA clone AC100A is shown in Figure 1 (EMBL/GenBank accession no. AF152103). Comparison with Related Sequences. The oISG17 cDNA sequence had 94% identity with the bISG17 cDNA (U96014) and exons 1 and 2 of the bISG17 gene (AF069133; Perry et al., 1999). It also shared 81% identity to human (h) ISG15 (M13755) and 80% identity with mouse (m) ISG15 (X56602) cDNA.